Is the nitrogen determination instrument an indirect method or a direct method?

Determining the protein content of rice and other food samples is an important method for judging the quality of food. For the convenience of modernization and effective improvement of the efficiency and accuracy of measurement, in many industries, nitrogen determination instruments are used for the determination. In general, protein measurement methods can be mainly divided into two categories, namely, indirect methods and direct methods, then the use of nitrogen determination of protein using indirect method or direct method?

Kjeldahl

Let us first look at what is the indirect method and the direct method. The indirect method uses the protein coefficient to calculate the protein content based on the nitrogen content after nitrogen determination. The direct method uses the physical or chemical properties of the protein and uses the instrument to determine the protein content by establishing a working curve. We know that the determinator is a professional instrument for the determination of protein in samples using the classical Kay method. The Dumas combustion method and the Kjeldahl method are the most widely used indirect methods. Therefore, we can know that the determinator measures protein The indirect method is used. So since the determination of protein by the nitrogen determination instrument is an indirect method, what is the direct method of determining protein? They are near-infrared spectroscopy, ultraviolet spectrophotometry, electrophoresis, ultraviolet absorption spectroscopy and Bradford method.

Although the nitrogen determination instrument uses the indirect method to determine the protein content of the sample, it has the advantages of high sensitivity, good stability, and low cost. With the development of science and technology, the degree of automation of the instrument is getting higher and higher, and the operation is more efficient. For simplicity and convenience, this method of analyzing nitrogen compounds has been highly respected in many industries and has become a statutory standard test method at home and abroad. At present, nitrogen determination instruments have been widely used in various industries. The instrument improves the analysis efficiency, saves test reagents, and enables rapid and accurate laboratory testing of large quantities of samples. It can be obtained in the shortest possible time. More accurate results have made outstanding contributions to the rapid development of many industries.

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